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Nannofossil analyses of Miocene to Pleistocene, section of Nglebur river, Jiken, Blora of Central Java included of Rembang Zone, North East Java Basin. This research have been performed on 70 samples collected from this section. The result of this analysis consist of 18 genera were obtained with 57 species of nannoplankton. The research method used of field study (measure section) and quantitative analysis. The samples from taken include fine-grained rock (marl, shale) and carbonaceous. The river section that have been selected consist of a continuous sediment sequence from Miocene (Wonocolo, Ledok and Mundu Formation) to Pleistocene (Lidah Formation). The sample preparation has been carried out using smears slide method and nannotex determination. The biostratigraphy consist of 11 zones, there are consist of 2 Partial Zones and 9 Hose Zones. These Zona are Discoaster hamatus and Discoaster neorectus Partial zone / NN9, Hoze Zone of Discoaster hamatus- Discoaster neorectus with Discoaster quinqueramus/ NN10, Zone of Discoaster quinqueramus - Ceratolithus armatus/ NN11, Zone of Ceratolithus armatus - Ceratolithus rugosus/ NN12, Zone of Ceratolithus rugosus-Discoaster asymmetricus/ NN12, Zona of Discoaster asymmetricus - Oolithotus fragilis and Pseudoemiliania lacunosa/ NN14, Zona of Oolithotus fragilis - Pseudoemiliania lacunosa with Discoaster pansus-Reticulofenestra pseudoumbilicus/ NN15, Zona of Discoaster pansus-Reticulofenestra pseudoumbilicus with Discoaster surculus/ NN16, Zona of Discoaster surculus - Discoaster asymmetricus/ NN17, Zona of Discoaster asymmetricus- Discoaster brouweri/ NN18, Partial Zone Discoaster brouweri and Gephyrocapsa oceanica/ NN19.