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Subclinical mastitis in Ettawa crossbreeds (PE) is an inflammatory disease that no clinical symptoms, but there is an increase the number of somatic cells and causes decrease milk production which economically detrimental. The aim of this study was to isolation of bacteria that causing subclinical mastitis in PE goats and their sensitivity with antimicrobial. A total of 37 PE goats from 5 farms in Kulonprogo were tested by California Mastitis Test (CMT). PE goats were said subclinical mastitis if the CMT test positive (++) or (++). Bacterial examination was carried out by enrichment in the peptone water buffer medium (BPW), and cultured in mannitol salt agar (MSA), eosin methylene blue agar (EMBA), and blood agar plate (PAD). Bacterial identification based on Gram staining, and biochemical tests such as confectionery. Subclinical mastitis in PE goats in Kulonprogo was caused by S. intermedius positive coagulase 4/4 (100%), S. aureus negative coagulase 4/10 (40%), S. aureus positive coagulase 3/10 (30%), and E. coli 1/10 (10%). S. intermedius positive coagulase was resistant to ampicillin, tetracycline, and sulfamethoxazole 2/4 (50%) respectively. S. aureus positive coagulase was resistant to ampicillin 2/7 (28.6%), penicillin 1/7 (14.3%), and sulfamethoxazole 1/7 (14.3%). S. aureus negative coagulase was resistant to ampicillin 3/7 (42.9%), penicillin 3/7 (42.9%), sulfametoxazole 2/7 (28.6%), and tetracycline group 1/7 (14.3%). This study showed that subclinical mastitis PE goats in Kulonprogo were caused by S. intermedius positive coagulase and S. aureus negative coagulase which are resistant to penicillin and sulfamethoxazole.