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The purpose of this study was to find out the results of the effectiveness of detergent detergency against stains on white cloth based on organoleptic tests and to determine the results of measurement tests for pollution levels with parameters of COD (Chemical Oxygen Demand), DO (Dissolve Oxygen) and pH levels in each each treatment. This research is an experimental study with a Completely Randomized Design (CRD) or Randomized Complete Block Design method, which takes 5 treatments, namely two types of control groups including negative control (K-) by adding distilled water and positive control (K +) by adding detergent contained on the market, and there are 3 treatment groups, namely P1, P2 and P3, respectively, Sanur Biodetergen with concentrations of 2.5%, 5% and 10% respectively. The instrument used for the extraction process is the Rotary Vacuum Evaporator and the instrument used for testing pollution levels is a set of titration tools with iodometric titration methods. The results obtained showed that the second treatment (P2) was the best treatment with an average detergency power of 3.10 or with faded parameters evenly with the edges of the stain that faded and had a minimum pollution level ie COD value 7.9 mg / L, DO value was 9.2 mg / L and pH level of 5.02, so that it qualifies as a low-contaminant liquid detergent that does not damage the environment.