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Background : Nutrition is an important factor in maintaining bone health, such as calcium, magnesium, phosphorus, protein, and iron. Sea cucumber is one sea creatures that has plenty of nutrition which good for the body. Objective : To compare the nutrition of Susu sea cucumber, Gosok sea cucumber, Gamat sea cucumber , and packaged milk so can be used as alternative source in bones health. Methods : The in vitro test using three samples of sea cucumber and three different kinds of packaged milk. The test was performed to five nutritions eg calcium, magnesium, phospor, protein, and iron. Analysis results using Independent Sample Test and Anova test. Results : Sea cucumbers can meet the number of daily nutritional needs. calcium in sea cucumbers is higher than milk (95% CI: -1969.85354 - 4094.26021). Phosphorus is higher than milk (95% CI: -224.68925). Magnesium is higher than milk (95% CI: 1499.67724 - 3243.20276). Protein is higher than milk (95% CI: 18.71876 - 67.70790). Iron is higher than milk (95% CI: 25.08204 - 54.45796). Conclusion : The nutrition in sea cucumber are greater than packaged milk. Susu sea cucumber better than the other two types. Sea cucumber could be used as alternative source for bones health.