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Soybean is very widely used in Indonesia, especially as a raw material for processed foods such as tofu and tempeh. This study aims to analyze the phytochemical content and proximate of variety irradiation soybean seeds Gamasugen 1 and Gamasugen 2. Phytochemical and proximate analysis on the seeds of soybean were carried out with quantitative and qualitative analysis. Proximate analysis analyzed included water content (oven method), ash content (furnace method), fat content (Soxhlet method), and crude fiber content (acid alkali digestion method). The results of phytochemical testing shows that both soybeans (Gamasugen 1 and Gamasugen 2) contain flavonoid as quercetin, alkaloid, saponins, phenolic, triterpenoid and glycoside. The proximate analysis results showed that Gamasugen 1 soybeans had a moisture content of 8.22%, lower than Gamasugen 2 which was 11.67%. Gamasugen 1 soybean content is 4.61%, lower than Gamasugen 2 which is 4.86%. Gamasugen 1 soybeans have a fat content of 14.50%, lower than Gamasugen 2 which is 15.51%. Gamasugen 1 soybeans have fiber content of 14.56%, higher than Gamasugen 2 soybeans which is 9.96%. The conclusion shows the highest proximate analysis of soybean is found in fat content (Gamasugen 2) which is 15.51% and the lowest level is found in ash content (Gamasugen 1) which is 4.61%.