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Waste of soybean husk seeds (SHS) is very potential organic waste to be used as organic fertilizer through vermicomposting techniques. To find out the potential, it is necessary to characterize the vermicompost metabolites produced, because by detecting metabolites it will be useful in studying the profile and its metabolic pathway. Gas Chromatography-Mass Spectroscopy (GC-MS) is used to detect metabolites because it is a powerful tool in characterizing vermicompost. The purpose of this study is to detect vermicompost metabolites made from raw materials of SHS waste and combination with cow manure. Thus, further information obtained can be used as a recommendation in handling SHS waste specifically as organic fertilizer through vermicomposting techniques. The study of vermicomposting was conducted using Randomized Block Design (RBD) with 5 treatments and 5 repetition. The treatment details are: V1: SHS waste (100%), V2: cow manure (100%), V3: SHS waste: cow manure (50%: 50%), V4: SHS waste: cow manure (75 %: 25%) and V5: SHS waste: cow manure (25%: 75%). Data were analyzed using GC-MS and Honestly Significant Difference (HSD) test. Based on the analysis using GC-MS in all treatments (V1-V5), there were detected metabolites classified as primary metabolites, consisting of: seven sugar compounds, 20 amino acid compounds, nine vitamins, three hormones and 43 organic acids. The treatment showed an increase in the chromatogram start of treatment V1-V5, and this result is directly proportional to HSD test where V5 treatment also showed the highest total curve. Key words: vermicompost, soybean husk, cow manure, GC-MS Abbreviations: SHS : soybean husk seed, GC-MS : Gas Chromatography-Mass Spectroscopy