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Centella asiatica is a popular plant that is currently the main claim for skin care ingredients that have the effect of accelerating the healing of zits and regenerating the skin. C. asiatica has a chemical component in the form of polyacetylenes, triterpenoids, asiaticosides, centellin, asiaticin and centellicin. The chemical components contained in C. asiatica have pharmacological effects in the form of antineoplastic, antiviral, antibacterial, antifungal, anti-inflammatory, antioxidant, anticonvulsant, antipsoriatic, anti-ulcer, wound healing, sedative, immunostimulant, cardioprotective, hepatoprotective, neuroprotective, antidiabetic, antidiabetic, and insecticidal . This many potential had made researcher explore the diversity of C. asiatica from various perspectives including DNA analysis. DNA profiling with SRAP has not been done, so this study becomes important data on C. asiatica. DNA profiling analysis of C. asiatica using SRAP with 8 primary combinations. Data was obtained from DNA isolation using the GeneJET Plant Genomic DNA Purification Mini Kit) or by modification. addition of PVP 2% of the total volume of lysis buffer A. Analysis using the Unweighted Pair method Method Group Using Aritmetic Method (UPGMA). Coordinate principle analysis (Principal Coordinate Analysis) of each individual. The data has been analyzed using a computer program (software) NTSYS 2.1, MVSP and PopGen 1.3 / GenAlex and other similar software. The results of the analysis showed that with SRAP marker C. asiatica formed 3 large clusters, cluster 1 had a 0.00 (0%) similarity coefficient, cluster 2 had a similarity coefficient value of 0, 12 (12%) and cluster 3 had a similarity coefficient value of 0.12 ( 12%). SRAP was able to determine the difference from 25 samples obtained from all over Indonesia.