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Introduction: The patients with type 2 Diabetes Mellitus who did not control their total cholesterol level properly ran the risk of various complications. Diet plan was important to prevent complications of the patients with Type 2 Diabetes Mellitus caused by hypercholesterolemia. Fasting on Mondays and Thursdays was appropriate with Sunnah of Prophet Muhammad SAW and potential for reducing the total cholesterol level of the patients with Type 2 Diabetes Mellitus. Method: This study used Quasy Experimental with pre test and post test control group design. This study was conducted on January to May 2016 in Kasihan, Bantul, Yogyakarta. The respondents consisted of 15 people in experimental group who were given intervention fasting every Monday and Thursday for one month with total sampling technique and 15 people in control group with standard treatment. The data was analyzed using Wilcoxon test and Independent T Test with significance p less than 0,05. Results: The average of cholesterol level after intervention was 189,87 reduce 21,52 in the experimental group and 223,33 reduce 45,77 in the control group. There were significant differences in the total cholesterol level between experimental and control groups before and after having fasting on Mondays and Thursdays and it showed that the total cholesterol level of experimental group was lower than control group, p result is 0,033. Discussion: Fasting on Mondays and Thursdays effectively reduced the total cholesterol level of the patients with Type 2 Diabetes Mellitus. Nurses could suggest the Diabetes Mellitus patients having fasting on Mondays and Thursdays with guidance ahead.