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The effectiveness of Education of Needlestick and Sharp Injuries to increase knowledge and attitude of Health Worker Kusbaryanto1 , Listiowati2 1 Department of public health Faculty of medicine and health Muhammadiyah University of Yogyakarta 2 Department of public health Faculty of medicine and health Muhammadiyah University of Yogyakarta Abstract Background: Nosocomial infection appears in a patient under medical care in the hospital. It is a worldwide problem that always increases significantly. It is necessary to apply standard precautions to minimize the risk of infection in hospitals. One of which is the risk of needlestick and sharp injuries. Objective: This study aims to find out the effectiveness of education about needlestick and sharp injuries module to increase knowledge and attitude of the health workers in hospital. Method: This study used quasi-experiments with pre-test and post-test control group design. To explore the knowledge about the risk of needlestick and sharp injuries, this study used purposive sampling with 29 respondents of control group and 103 respondents of treatment group. To explore the attitude of the risk of needlestick and sharp injuries, this study used purposive sampling with 28 respondents of control group and 98 respondents of treatment group. The data were analyzed by Wilcoxon and Independent sample T test. This study used questionnaire as an instrument to collect the data. Result: The result showed that knowledge about the risk of needlestick and sharp injuries in control group is p = 0,578 (p > 0,05), which is not significant. The result from treatment group is p = 0,001, which is significant. There is a significant difference between control and treatment group in comparison. The result showed that attitude of the risk of needlestick and sharp injuries in control group is p = 0,277 (p > 0,05), which is not significant. The result of treatment group is p = 0,001, which is significant. There is a significant difference between control and treatment group in comparison. Conclusion: The education is effective to increase knowledge and attitude of the health workers about the risk of needlestick and sharp injuries in hospital. Keywords: Effectiveness, Education of risk of needlestick and sharp injuries, knowledge, attitude, health worker