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E-Commerce has a huge role in the current era of the 4.0 Industrial Revolution, but still few business actors are able to adopt an e-commerce system. E-commerce e-Siatik with domain www.ternakitik.com has been created as a container for both duck farmers and SMEs of salted egg to conduct digital buy and sell transactions. This research aims to test the readiness of e-commerce implementation (e-Siatik) for duck farmers as well as SMEs of salted egg in Brebes Regency using the Technology Acceptance Model (TAM) approach. The variables used are perceived usefullness, perceived easy to use, computer self efficacy, attitude towards using, and intention to use. Sampling is done using the convenience sampling technique. Data analysis by adopting the Technology Acceptance Model (TAM) approach and statistical tests using Structural Equation Modelling (SEM) techniques. The results of the test indicated that perceived usefullness affect on the attitude towards using and intention to use, computer self efficacy affect on the perceived ease of use and perceived usefullness, attitude towards affect on the intention to use, as well as intention to use affect on the actual usage.