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This research is carried out due to the limited information on the shelf-life of food product in Indonesia, especially in South Sulawesi. In addition, there is no sufficient information on the self-life of packed seaweed dodol which produced by using scientific method. Seaweed dodol has a high economic value. The objective of this research was to estimate the self-life of seaweed dodol which is produced in traditional scale (micro, small, and middle scale bussiness) using the scientific method. Accelerated Shelf Life Testing (ASLT) Method is one of methods used in determining the shelf life of seaweed dodol based on critical water content and graining as indicators of quality deterioration. The isotermic sorption model of products was obtained from isotermic sorption curve formed from the relationship between the values of water activity (aw) and the equilibrium moisture content (Me). Moreover, five different models of isotermic sorption were tested. They were Hasley, Chen-Clayton, Henderson, Caurie, and Oswin model. The results showed that the Henderson model was the appropriate model in describing the isotermic sorption phenomena at seaweed dodol with the Mean Relative Deviatian (MRD) of 1.4. By integrating the critical moisture content (Mc), packing permeability (OPP), sample weights (Ws), the surface area of packing (A) and the saturated vapor pressure (Po) into the equation Labuza, the self-life of seaweed dodol at temperature of 30 oC and RH of 78% was 56 days.