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Glucose is one of the most important substances for medical check-up. Therefore, many studies are being done to find technology that can carry out research-level analysis and fast way to detect glucose. Making a portable sensor that can properly support the results of sample testing is a solution to this problem. Screen-printed carbon electrode (SPCE) is a common portable strip sensor based on electrochemical method. However, the working electrodes from carbon-based SPE must be modified with additional material to improve the performance. Many factors determine the modification such as the deposition method and the material itself. In this study, the working electrode of SPCE was modified by making a film through simple drop-casting method. From the experiment, SPCE that modified with carbon nanomaterial paste (Graphene-Polyurethane) show performance improvement for electrochemical analysis, but not enough to detect glucose. Thus SPCE was modified by cobalt metal organic framework (Co-MOF) nanomaterial to be able to detect glucose non enzymatically. It has a characteristic error measurement of 1.7% and selective to glucose when compared with urea and NaCl. This data is obtained with electrochemical testing such as cyclic voltammetry (CV) and differential pulse voltammetry (DPV).