Indonesia Conference Directory

Abstract. Secondary metabolite of epigallocatechin gallate is a polymeric ester compound from epigallocatechin and gallic acid. This secondary metabolite of epigallocatechin gallate is found in the Camellia sinensis L plant which is bioactive that can be an antioxidant and capable to prevent cancer. The problem of the epigallocatechin gallate-s existence from plants depends on: the height of the land from the sea level, the condition of the environment temperature,and it requires an intensive care and its production level is relatively low. Therefore, the technology of production of epigallocatechin gallate is needed through in vitro culture techniques. This technique is environmentally controlled, is effective in cultivation and only requires a limited area. The purpose of the study was to obtain a secondary metabolite production technique of epigallocatechin gallate through in vitro culture by optimizing the medium and giving precursors. The method to reach the research objectives are: (1) initiation of callus by immersing the explants from leaf pieces on the growth medium that is being added by growth regulating hormones. (2) Stimulating callus culture by giving cinnamic acid precursors. (3) Callus growth testing. (4) Qualitative testing of the epigallocatechin gallate morphology from callus. (5) Quantitative testing of epigallocatechin gallate from callus. The conclusion of this study is that callus products that are composed of epigallocatechin gallate are produced. The implication of this study is that the use of cinnamic acid precursors in the in vitro culture technique of Camellia sinensis over a relatively short period of time can increase callus wet weight