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Abstract. Colloidal Silver Nanoparticles were synthesized by Gamma ray irradiation in aqueous lactic acid solution containing chitosan as stabilizer. The aim of this research is to know the effect of irradiation dose and concentration of chitosan in the formation of Nano-silver. Silver Nitrate of 2 mM is dissolve in the chitosan solution. The effect of chitosan concentration which varied 0,.3%, 0. 5%, and 0. 7%(b/v) were irradiated with gamma ray at dose of 5, 10 and 15 kGy. The formed of silver Nano were identified using UV –Vis spectrophotometer, particle size analyzer (PSA) and FTIR. The result shows, the concentration of chitosan (0.3 – 0,7%) in the irradiated solution also considerably affected the silver Nano particles size which decreased with increasing of irradiation dose. Due to the unique features of the processing procedures, thus Co-60 ray irradiation has been considered as a suitable method for synthesis of colloidal silver nanoparticles.