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Abstract The aims of this study were to evaluate survival and morphological performances of tiger shrimp P. monodon after methanol/water (1:1) extracted Sargassum duplicatum immersion under different dosages. Four treatments were applied during this study, which were 600 (A), 700 (B), 800 (C) and 0 ppm (D) as control. Samples of PL-4 of tiger shrimp were immersed then the survival rates were calculated for each treatment. The number of post larva for each treatment was 6 individuals. The morphological performances of larvae were observed under microscope to access antennules, hepatopancreas, front gut, midgut, uropods, tail muscle, chromatophores, fouling and stress appearances. The “+” sign was recorded for larvae with normal morphological performances; in contrast the “-“ sign was recorded for samples with non-normal performances. The results of this study showed that the immersion of tiger shrimp post larvae in methanol:water extracted Sargassum duplicatum did not significantly affect survival rates of tiger shrimp post larvae. Morphological performances of tiger shrimp post larvae observed in antennules, front gut, midgut, uropods, tail muscle, chromatophores, fouling and stress appearances were similar among all treatments. In contrast, hepatopancreas showed anomaly between treatments indicating Sargassum duplicatum contain bioactive to combat microorganisms cause diseases on tiger shrimp.