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Damping off is one of the diseases caused by soil borne pathogens that cause the death of seedling in a short time due to decay in seeds or roots and stems. Control of this disease is quite difficult, so control measures must be taken before a pathogen attack occurs. One potential for controlling this disease is the use of biofertilizers. The purpose of this study was to examine the potential of biofertilizers and the time of application to the intensity of damping off disease (Fusarium sp.) and plant growth on Japansche citroen seedling. This study used a Factorial Completely Randomized Design with 2 factors. The first factor was the type of biofertilizer namely Azozfor (P1), liquid biofertlizer (P2), secondary metabolite Trichoderma asperellum (P3), Mixtured of three biofertilizer (P4), and control (P5). The second factor was the time of application, namely the treatment of biofertilizers first then followed by the inoculation of Fusarium sp. (A1) and the treatment of Fusarium sp. first followed by treatment of biofertilizer (A2). Observation parameters were the intensity of the disease, seeds germinate and plant height. The results showed that the intensity of the lowest germination rate was found in A1P3 treatment (0%), while in the control of the intensity of shoot sprout disease reached 41.3%. The treatment of A1P2, A2P1, A2P2 and A2P4 showed the same disease intensity of 1.3%. The highest control effectiveness was found in A1P2, A1P3, A2P1, A2P2 and A2P4 treatments and this was not significantly different compared to A1P4, A2P4 and control treatments. The largest percentage of seeds germinate at 21 days after seedling (DAS) was shown in A1P1 treatment with a percentage of 26.7% and A2P3 treatment (25.3%), while after 28 DAS, the largest percentage of seed growth was in A2P4 treatment with percentage of 73.3%. The lowest plant height at 28 DAS was A1P4 treatment and control. Biofertilizer is effective for controlling damping off disease if applied separately.