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Bacterial growth media have a diversity of nutrients. Culture media is very important for isolation, testing the physical and biochemical properties of bacteria and for diagnosing a disease. The present study aimed to examine the effectiveness of soybean seed sprouts, green beans, red beans, and peanuts, whether it has the potential to grow the breed Gram positive and Gram-negative bacteria. The research method, nut bean seeds were washed, germinated, dried in an oven at 40oC, blended and sieved in term to obtain flour. Flour was extracted to obtain extract solution plus agar powder and broth was made in agar media in petri dishes as well as formulas and compositions on Nutrient Agar (NA) media as a comparison standard. Then the media planted with Staphylococcus aureus and Escherichia coli bacterias, it was incubated at 37oC for 1-3 x 24 hours, observed the growth of bacterial colonies, counted the number of colonies, and measured the diameter of the colony as research data. As a comparison, NA media was made and the same test bacteria were planted. The analysis of the percentage of the effectiveness of the growth of Staphylococcus aureus bacteria on various bean seeds media compared to Nutrient media: soybean medium 76.67% (less effective); mung bean 75.71% (less effective); red bean 101.79% (very effective); and peanut 99.53% (effective); whereas for Escherichia coli bacteria are: soybean 97.29% (effective); green beans 93.75% (effective); red beans 88.89% (quite effective) and peanuts 122.22% (very effective). In conclusion, various growth bean media can grow Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria Escherichia coli. Growth media of various bean seeds are effective in growing but not effective in breeding S. aureus bacteria. Growth media of various bean seeds are effective in growing and breeding E. coli bacteria which are capable of replacing Nutrient Agar (NA) media.