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Biology learning requires students to carry out observations in practicum activities to form of concept understanding. Based on the observations in class XI of SMAN 3 and SMAN 7 Padang, it was found that the school had carried out practicum activities, but students had difficulty in practicing, especially plant tissue. They did not find clear observations, less skilled in using microscopes and making plant preparations. In the other hand some microscopes had moldy lenses, and the unavailability of practical guidance media that contained photographs of plant tissue observations slashed manually, so that the plant tissue observations carried out did not go smoothly. This development research using tree stages from the 4D development model, namely definition, design and development. The research subjects were 28 students, one teacher, and two lecturers. The instruments were used interview guides, validity and practicality questionnaires. Based on research conducted, have been produced Plant Tissues Atlas for SMA / MA. The validity test results obtained an average value of 3.73. with very valid criteria from the aspects of the appropriateness of content, language and readability, and presentation. Practicality test results obtained an average value of 3.81 with very practical criteria by teachers and 3.65 with very practical criteria by students in terms of learning activities, ease of use, and benefits of use. Based on these, it can be concluded that the Plant Tissues Atlas developed is very valid and very practical