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Eucheuma spinosum is one of seaweed species that can be found abundantly in Takalar Regency. The biomass of this seaweed has been used to remove Mn(II) ion from the aqueous solution via the adsorption process using E.spinosum. The adsorption of Mn(II) ion was studied in various contact time, pH and concentration. An Atomic Absorption Spectrophotometer (AAS) was an instrument used to determine the amount of ion before and after adsorption. Langmuir and Freundlich equations were used to study the adsorption isotherms of the ion. The results showed that the optimum time obtained was 20 minutes and the optimum pH was 4. The Freundlich isotherm fitted the adsorption of ion Mn(II) using E. spinosum with the capacity (Qo) of 9.07 mg/g. The functional group involved in biosorption of Mn(II) metal ions was hydroxyl (-OH) groups.