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This study aims to determine and explain the role of organizational commitment in mediating the effect of motivation and organizational culture on the performance of employees at Cooperative in Badung regency. The method used in this research is quantitative with 97 people as sample calculated by slovin formula. To determine the sample size used probability sampling method. The data used in this study are primary and secondary data both quantitative and qualitative data. The results showed that motivation had a negative and not significant effect on employee performance as well as the organizational culture that had a negative and a not significant effect on the performance of employees at the Cooperative in Badung regency. However, in terms of organizational commitment, this study found a positive and significant influence on employee performance and fostered a positive motivation. The organizational commitment is the perfect mediation in mediating the organizational culture of employee performance at the Cooperative in Badung regency.