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The Indonesian Ministry of Health responsible for the supply and distribution of logistics needed in the implementation of the Immunization Program at national, provincial, district, and health facility levels. This study discussed cold chain management of vaccines from Provincial Health Office to the health center facilities and tested the vaccine potency. We have used a cross-sectional study involving a multistage sampling. Primary health centers, clinics and hospitals were selected from each two districts of eleven provinces. The data were analyzed descriptively and analytically using Chi Square Test. Vaccine potency test was carried out by Kendrick test. This study confirmed that the cold chain management of vaccines at the provincial level was better than the districts and health center facilities. The quality of Vaccine Vial Monitor (VVM) was in gradually decline from provinces to districts and health center facilities. Potency test results of DPT and polio vaccines suggested that all vaccines are still qualified in all study sample locations.