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The study was conducted to determine the role of pulsed electric field (PEF) as pre-treatment on the extraction of phenolic compounds from basil (Ocimum americanum L.) leaves. The research used Randomized Block Design (RBD) with 2 factors, factor 1: exposure times (10, 15, and 20 seconds) and factor 2: voltages (1000, 1500, and 2000 volt). Extraction was done by maceration using 80% ethanol with temperature of 50oC. The results showed that PEF treatment had an effect on total phenolic compounds (TPC), phenol yield and antioxidant activity (IC50) of basil extract. The sample treated at exposure time of 10 seconds and voltage of 1500 volt obtained TPC of 260.50 mg GAE/g, phenol yield of 1.91 % and IC50 of 35.96 ppm. Whereas the untreated sample (without PEF) obtained TPC of 193 mg GAE/g, phenol yield of 0.86 % and IC50 of 166.78 ppm. The data showed that PEF treatment before the extraction of basil leaves can increase total phenolic compounds by 1.4 times, phenol yield by 2.2 times, and antioxidant activity by 4.6 times.