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Our study about chromosomes imaging using DAPI stimulating light and soda lime glass microspheres whose diameters are spread from 35 μm to 120 μm shows that the magnification power of this system is 3 to 5 times. Images of chromosomes that colored with DAPI beneath the microspheres are observed have a size 3 to 4 times larger than the actual chromosomes. In addition, using microsphere lens approximation, the magnification power of chromosomes image without DAPI is 3.8 to 12.6 times whereas the magnification power of DAPI-stained chromosomes is 3.7 to 4.8. The spherical lens approximation does not show any variation of magnification even though the microsphere’s diameter is changed. Experimentally, there is an evident that microspheres can improve the performance of microscope in chromosomes imaging. This system is potential to engineer the optical microscope by applying photonics jet phenomenon.