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Mining is one of the leading industrial sectors in Indonesia but has a negative impact on the environment. Acid mine drainage (AMD) is one of the negative impacts of mining activities that can cause environmental problems and require efficient and effective treatment. The development of acid mine drainage treatment involves the use of microalgae for remediation. The problem with the use of microalgae in AMD is the environmental conditions of AMD that limit the growth of microalgae where AMD has a high metal content, and low pH. This research was conducted to examine the adaptability of Botryococcus braunii microalgae on AMD media. The study was conducted on photobioreactors by varying the concentration of AAT at 25%, 50%, 75%, and 100% to assess the growth rate and removal efficiency of Fe and Mn metals as well as the pH increase in AMD. Botryococcus braunii is expected to be able to adapt gradually to the AMD media, so that AMD can become a growing medium for the microalgae. The output of this research is expected to be able to give an idea of the possible application of microalgae to biological AMD treatment.