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In present study immobilized cells of bacterial Pseudomonas putida was used as biosorbent for the removal of Pb2+ ions from aqueous solution. Pseudomonas putida was immobilized with 3% sodium alginate and 2% CaCl2. The adsorption studies of bacterium was investigated in batch mode. Optimum biosorption conditions were determined as a function of pH, different concentration of Pb2+ ions and contact time. The equilibrium adsorption data was analyzed by the Langmuir and Freundlich isotherm models. The value of regression coefficient of the isotherm represented the best fit of R2 0,94 with the Langmuir isotherm. The maximum monolayer adsorption capacity (qe) of the biosorbent, as obtained from the Langmuir adsorption isotherm was found 54,05 mg/g with Langmuir adsorption constant (k_L) 0,05 L/mg, respectively at 90 minutes contact time and pH 5. Kinetics of adsorption was analysed by pseudo first order and pseudo second order. Kinetics of adsorption followed pseudo second order with regression coefficient of pseudo second orde of R2 0,961 ,the coefficient of rate constant for pseudo second order (k_2) obtained 0,4 g/mg.min, pointing that the adsorption mechanism is chemical and physical adsorption process.