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The garlic (Allium sativum L) belonging to the genus Allium , propagated in vegetative through bulb. In the plants propagated by vegetative technique virus is an important disease. The tissue culture techniques in combination with chemotheraphy could be eliminate virus diseases. The experiment carried out in the laboratory tissue culture of IVEGRI. The experiment aims to observe effect of several antiviral Ribavirin concentration in MS medium on growth and development Shoot tip cv. Lumbu hijau, cv.Lumbu putih, cv. Tawangmangu, to produce virus-free plant. Media composition, were : R1 until R4 that is : MS + MS vits + sucrose 30 g / l + IAA 2 mg / l Kinetin + 2 mg / l + GA3 0:01 mg / l + gelgro 2 g / l + ( Ribavirin 0 , 5, 10, 15 mg/l), pH 5.7. The results of the experiment a) Culture contamination were caused by bacteria and fungi with a percentage of 25 to 55 %. b). In high concentration of antiviral Ribavirin gave results on decreasing growth and development of three cultivar garlic. c). On visual observation cultivar and added of antiviral Ribavirin has no effect on the number of shoot. d). The added of antiviral Ribavirin and cultivar does not affect on growth three cultivar garlic. e) The virus test by serological DAS ELISA, percentage of infected plantlets were 54.55% to 100%.