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Bamboo is a natural material that in a great demand in the field of simple construction, so connection is one of the things that need to be considered. Research on bamboo connection has been carried out using bolts, steel gussets with wood or mortar fillers, and Fiber Reinforced Polymer (FRP). The use of FRP has advantage since FRP is light, strong, and has adequately high stiffness. This research uses two types of perpendicular connecton to fiber (TLS) with two layers methods, different number of layers, and two bolts which function as lateral connection. The used connection testing method is quasi-static monotonic loading. From the results of the study it is found that TLS01.2L increased the maximum load by 80,05 percent compared to TLS01.1L, while in TLS02.2L there is a decrease of 20,65 percent compared to TLS02.1L. In addition, the average ductility value is 2,63 for TLS01 and 2,11 for TLS02 so the stiffness value only reaches 1,75 for TLS01 and 1,97 for TLS02. It can be concluded that the numbers of layer does not affect the strength of the TLS connection but rather the FRP bonding area and the windings method which influences the strength and stiffness of the connection.