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The purpose of this study is to apply to learn using Augmented Reality (AR) to improve the spatial ability of alkane and cycloalkane conformation. The method of this study quasi-experimental control group pretest-posttest design for the second-semester students of the Chemical Education at UIN Sunan Gunung Djati Bandung. Data obtained by instruments to measure student activity during learning, completion of students on student worksheets, and pretest-posttest results for spatial abilities. Data collection techniques were carried out with tests, while data analysis was carried out with statistical methods with the help of SPSS version 23. The results obtained in student activities were categorized as very good (mean 86.3%), while the results obtained from the completion of LK were categorized very well ( mean 84.53), the results of the spatial ability test show an increase in spatial ability with N-gain results classified as moderate (mean 0.58). This study shows that the application of alkane and cycloalkane AR conformation can visualize the movement of an object that is abstract, thus increasing spatial ability. Therefore the implementation of alkane and cycloalkane AR conformation can improve the spatial ability of students.