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Introduction: Malathion is one of organophosphate pesticides that can cause intoxication when used inappropriately. Pulutan (Urena lobata) is medicinal plant used to treat some diseases empirically and pre-clinical study has already proven its efficacy as antioxidant and anti-inflammatory. The study aims to evaluate the potential of Urena lobata (U. lobata) leaf extract on blood glucose level and hemoglobin (Hb) in the juvenile and adult of zebra fish (Danio rerio) exposed by malathion. Methods: The object study using juvenile and adult of zebra fish (Danio rerio) which divided into each 2 control group and 3 test group (n=4). The leaf of U. lobata was extracted by decoction methods therefore it was diluted into 125 mg/L, 250 mg/L and 500 mg/L. The animal test was exposed by herbs for 40 days concomitant with malathion 5 mg/L. Blood glucose level and Hb were measured using a commercially available glucometer and Hb meter. All data are expressed as the mean ± SD and analyzed with one-way anova and continued with LSD (p<0.05). Results: The administration of U.lobata leaf extract at dose of 125 mg/L and 500 mg/L able to inhibit the decrease of Hb level more and less 10 % and 20 % respectively compare to malathion group (p<0.05) both of on juvenile and adult of zebra fish (Danio rerio). Whereas the blood glucose level was decreased by 40%, 60% and 40% (p<0.05) respectively on juvenile that were given U. lobata at dose of 125 mg/L, 250 mg/L and 500 mg/L, meanwhile on adult of zebra fish were reduced 60%, 50% and 50% respectively (p<0.05). Conclusion: U. lobata leaf extract is able to inhibit the increase of blood glucose level and the decrease of Hb level both of on juvenile and adult of zebra fish (Danio rerio).