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The growth of animals can be determined by various hormones, one of which is somatotropin. Somatotropin has remarkable effects on the tissue growth. This study aims at examining the effects of somatotropin on male rate growth performance. The samples were one hundred and sixty two 2-week old Sprague-Dawley male rats. Data analysis was performed using two-factor nested designs. The first factor was dosage consisting of 3 levels (0, 3, and 9 mg/kg bw/day). The second factor was the injection period consisting of 3 injection periods (day 1 to day 14, day 1 to day 28, and day 15 to day 28). The results showed that during day 1 to day 28 of the injection period, somatotropin improved bone length and bone composition and that no further growth after day 28. This may imply that somatotropin could improve the growth of male rats.