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Background: Professional behavior is the key success for nursing career as professional nurses. Nursing education play important role to educate nursing student act accordingly to tackle the complex demand in health care environment. However, the instrument to measure professional behavior among nursing student remains scarce. Objectives: To adapt and validate the Nursing Students Professional Behavior Scale (NSPBS) through pilot study to measure professional behavior among Indonesian nursing students. Method: The original instrument (27 items) underwent the guideline of translation and adaptation instrument by World Health Organization (WHO) (2018). The total sample of study is 364 nursing students who ever exposure clinical practice in hospital. The participants were students from Bachelor-level nursing school at private University in Yogyakarta (Indonesia) based on their responses to the Indonesian version from August-September 2018. A Confirmatory Factor Analysis (CFA) using Partial Least Square (PLS)-Structural Equation Modelling (SEM) were conducted to evaluated the validity and reliability. Results: The data had fit for three-factor model including healthcare practice, activity practice and reporting. The face validity and content validity has evaluated by six experts. The CFA showed excellent result with factor loading varying between.70 to .96 for the constructs. While the composite reliability (CR) for all latent constructs range from .90 to .96, which means that the internal consistency reliability was high. Conclusion The translation, cultural adaptation and validation process of the NSPBS Indonesian version are resulted 27 items in three subscales. Indonesian version of NSPBS instrument are valid and reliable so it could implement to measure professional behavior of nursing students.