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The adequacy of a persons iodine is usually assessed by the level of urine iodine. However, the level of urine iodine has not been able to describe the levels of fT4 and TSH. This study was to determine the relationship of blood iodine levels with fT4 and TSH serum level of children aged less than 2 years in endemic areas of IDD. Blood iodine level was measured using Ammonium Persulfat Dry Digestion method and fT4 and TSH using ELISA method in 50 children under the age of 2 years in Ngargosari Samigaluh Village. Spearman correlation test was used for statistical analysis. There were 44 children (88%) with blood iodine levels 52 until109 μg / L, having a mean fT4 of 1,638 more or less 0,163 ng / dL and a mean TSH of 2,619 more or less 2,982 mIU / L. Subjects with a blood iodine level of less than 52 μg / L totaled 4 children (8%) with a mean fT4 of 1,605 more or less 0,161 ng / dL and a mean TSH level of 1,865 more or less 1.17 mIU / L. Subjects with blood iodine were more than 109 μg / L totaling 2 children (4%) with a mean fT4 level of 1.69 +0.084 ng / dL and a mean TSH level of 4.16 more or less 0.82 mIU / L. The blood iodine content of children in Ngargosari Samigaluh Village did not significantly correlate with fT4 hormone levels (p = 0.7690) or TSH (p = 0.287)