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Sexually mature Indonesian native hens (Sentul hens) were housed singly in laying cages and artificially inseminated with combination of three different levels of diluted pooled semen (50 million sperm/0.05 ml; 100 million sperm/0.1 ml; and 150 million sperm/0.15 ml) and at either of three different intervals (every 3, 6 and 9 days). The results show that the sperm number and Insemination inverval had no significant interaction (P>0.05) on % fertility and hatchability. The best fertility (P< 0.05) was obtained by inseminating interval 6 days with sperm number 100 million/ 0.1 ml of diluted semen.