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Bacterial infection and injury caused by dressing removal are two concerning issues affecting the healing process in treatment of skin damaged. Electrospun nanofiber as substrate demonstrates promising results in tissue recovery process by promotion of cell adhesion and expansion. In this study, the electrospun of polyvinyl alcohol/poly-ε-caprolactone (PVA/PCL) nanofiber coated with two different types of natural antibacterial agents, i.e. poly-L-lysine and chitosan were investigated. PVA-PCL coated antibiotic chlorhexidine gluconate (CHG) was also prepared for comparison purpose. The FESEM images showed an increase in fiber diameter with the presence of antibacterial agents. The effects of antibacterial agents were examined by Disk Diffusion Test against S.aureus and E. coli based on measurement of inhibition zone of bacterial growth. The results reveal that CHG, poly-L-lysine and chitosan started to inhibit the growth of bacteria when coating in 5, 15 and 20 min, respectively. Besides, the tensile strength values of PVA/PCL/antibacterial agents tested appear to be within the range of tensile strength values for human skin which conclude that the PVA/PCL/antibacterial agent nanofiber is suitable for regenerating human skin. Lastly, the hydrophilicity of the electrospun of PVA/PCL/antibacterial agents nanofiber was confirmed by water contact angle measurement which is 0°, thus theoretically can improve the cell adhesion and expansion onto the skin membrane.