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Study of the mercury (Hg II) removal from aqueous solution has been investigated by adsoprtion using a low-cost bentonite nanoparticle. The nano-bentonite adsorbent was prepared by heating method at temperature of 400 Celcius. The activation was carried out by immersing natural bentonite in sodium hydroxide. The resulting powder was heated in the present of nitrogen gas as an activator agent. The bentonite sample was characterized by means of Fourier Transform Infra-Red (FTIR), Scanning Electron Microscopy (SEM), and X-Ray Diffractometer (XRD). The prepared bentonite had a heterogeneous surface with a particle size of 300 nm. The nano adsorbent was tested in the absorption of synthetic Hg(II) solution which concentration varied from 5 to 120 ppm with 0.1 gram absorbent and volume of 100 ml. The adsorbed amount of Hg(II) was analyzed using Atomic Absorption Spectroscopy (AAS). The adsorption capacities were fully fit with Freundlich and Langmuir isotherm models. It was found that the adsorption process followed the Freundlich isotherm model with and adsorption capacity of 6.086 mg/g. Therefore, we conclude that nano-bentonite can be a potential low-cost adsorbent for removal of mercury in water.