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ALTERATION OF THE MITOCHONDRIA MARKER INDUCED BY AEROBIC EXERCISE SERUM IN H9C2 CELL LINE
Wibowo Budi Prasetyo(1), Vita Murniati Tarawan(2),, Ronny Lesmana(2,3), Hanna Goenawan(2,3), and Unang Supratman(4)

1. Undergraduate Program Medical Doctor, Faculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
2. Physiology Division, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
3. Physiology Molecular Laboratory, Biological Activity Division, Central Laboratory, Universitas Padjadjaran, Jatinangor 45363, Indonesia.
4. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jatinangor 45363, Indonesia.


Abstract

Exercise-induced hearth muscle adaptation for under stress conditions, known as Mitochondria Quality Control. Each stage of Mitochondria quality control, has a coactivator as a trigger for the process. Where it starts from biogenesis with PGC-1α , After that, it will enter the fusion-fission cycle stage which are mediated by Mfn1 and Mfn2 for fusion in the outer membrane and Opa1 for fusion in the inner membrane. After fusion, fission occurs in the old and unhealthy parts mediated by Drp1. Finally, the old and unhealthy mitochondrial section occurs mitophagy mediated by PINK-PARKIN signaling pathway. The aim of this study was to determine the difference effect of PGC-1α, Mfn1, Mfn2, Opa1, Drp1, PINK, and Parkin expression in aerobic physical activity for Mitochondria Quality Control in cardiac muscle. This is limited because only rats are used. 12 male wistar rats were assigned to 2 groups: sedentary control and Aerobic-intensity (AE, 15m/minute). Training was conducted 30 minutes/day with a 5 times/week interval for 8 weeks. On the last day, control and exercise groups were taken blood serum from the retro-orbital sinus for culture of H9C2 Cell line, and extract RNA and protein. The expression of the PGC-1α, Mfn1, Mfn2, Opa1, Drp1, PINK, and Parkin gene in cardiac muscles was then amplified using reversed PCR and detected by agarose gel electrophoresis. This study concludes that different effect of PGC-1α, Mfn1, Mfn2, Opa1, Drp1, PINK, and Parkin gene expression level were increased level affected by aerobic physical activity in cardiac muscle.

Keywords: Aerobic Exercise, Mitochondrial Quality Control, H9C2 Cell Line PGC-1α, Mfn1, Mfn2, Opa1, Drp1, PINK1, Parkin

Topic: Biomarker, Drug Design, Delivery and Teragnostic

Link: https://ifory.id/abstract/Xztm4cTvHnbZ

Conference: The 1st Bandung Applied Biomedical and Technology in Health Conference (BABTECH 2019)

Plain Format | Corresponding Author (Wibowo Budi Prasetyo)

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