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The purpose of the experiment was to study the effect of gibberellin (GA3) concentration on the growth of sugarcane orchid (Grammatophyllum speciosum) protocom in vitro. The experiment was conducted at the laboratory of Winaya Mukti University, from May 2018 until August 2018. The design was used a Completely Randomized Design (CRD) with five treatments and five replications. The concentrations were tested : A = 0 mg L-1 (without GA3), B = 5 mg L-1 solution of GA3, C = 10 mg L-1 solution of GA3, D = 15 mg L-1 solution of GA3, E = 20 mg L-1 solution of GA3. The result of the experiment showed that the concentration of gibberellin (GA3) have an effect on the growth of sugarcane orchid (Grammatophyllum speciosum) protocorm in vitro. Giving 5 mg L-1 solution of GA3 have an effect on the plant height of 12 WAC (weeks after culture).