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Phlebia sp. MG-60 is one of white rot marine fungi produced enzyme MnP on the hypersaline condition which could potentially degrade lignocellulose especially lignin and to produce ethanol directly through integrated fungal fermentation. This study aimed to determine the effects of the addition of MnSO4 and sea salt on the degradation of lignocellulosic compounds and ethanol production using Phlebia sp. MG-60. The results showed that the addition of MnSO4, sea salt, and their interaction significantly affected on the change of weight loss, total soluble phenol (TSP), total reducing sugar (TRS), pH, lignin concentration, and bioethanol concentration. The best treatment was obtained from the addition of MnSO4 300mg/l, sea salt 3% and 20 days of incubation time. This treatment has the highest value of TRS (22.471 mg/g), TSP (0.334 mg/g), weight loss (16.23%), and ethanol (0.1%), with the lowest value of lignin (19.17%) and pH (6.1). The present results indicated that suitable culture conditions could significantly improve integrated fungal fermentation.