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Abstract. Biodiesel was produced from Kapok seed (Ceiba Pentranda) oil (KSO) using heterogeneous bimetallic oxide of copper and zinc supported by γ-alumina. The Alumina-supported copper oxide- zinc oxide (CuO- ZnO γ /- Al2O3) further notated by ACZ was synthesized using precipitation, impregnation and gel methods. It also was characterized using the X-ray diffraction (X-RD), Brunauwer-Emmett Teller (BET), and scanning electron microscopy-EDX (SEM-EDX) methods. Before being transesterified the KSO was pre-treated to reduce gum and free fatty acid content. The activity test of ACZ catalyst was done by introducing it through the transesterification process of KSO with methanol. The transesterification process was conducted in a glass batch type reactor with refluxed methanol. The effect of loading ZnO (wt. %), calcinating temperature (°C), and calcinating time of catalyst (h) on the yield of biodiesel were investigated, respectively. The results showed that the kapok seed oil was worthy and potentially to transesterified into biodiesel using ACZ catalyst.