Transformation and Activity evaluation of Sesame Seed Oil (Sesamum Indicum Linn) Derivatives as Antimicrobial Agent Sutrisno1, a), Ihsan Budi Rachman1, Dedek Sukarianingsing1 and Ainul Yaqin1
1Chemistry Department Faculty of Mathematics and Natural Sciences Universitas Negeri Malang Jl. Semarang No. 5 Malang 65341
a) Corresponding author: sutrisno.kimia[at]um.ac.id
Abstract
Sesame seed oil is a vegetable oil sourced from sesame seed extract. Sesame seed oil is rich with unsaturated fatty acids, especially oleic acid (C18:1 9c) and linoleic acid (C18:2 9c12c, ω-6). Sesame seed oil has not been widely utilized in the field of pharmacy, because it is expensive and more used for cooking. Previous studies show that sesame seed oil had no antibacterial activity and was acquired the fatty acid constituent components of sesame seed oil. The research aims are: (1) Transformation sesame seed oil into its derivatives, (2) characterization the sesame seed oil derivative products (3) identification of the product of sesame seed oil and (4) evaluation of antibacterial activity of its derivatives. The research consisted of 5 stages. Stage 1: characterization and identification of sesame seed oil which includes appearance, color, density, refractive index, viscosity, solubility test, acid number, number of conformations, ester number, IR spectrum interpretation and GC-MS. Stage 2: synthesis, characterization and identification which includes the appearance, color, melting point, solubility test and IR spectrum interpretation of potassium soap. Stage 3: synthesis, characterization and identification which include form, color, density, refractive index, viscosity, boiling point, solubility test, acid number, number of conformations, ester number and IR spectrum interpretation of fatty acids. Stage 4: transesterification of sesame seed oil, characterization and identification which include appearance, color, density, refractive index, viscosity, solubility test, acid number, number of conformations, ester number, IR spectrum interpretation and GC-MS. Stage 5: evaluation of antibacterial activity to Staphylococcus aureus and Escherchia coli bacteria. The saponification of sesame seed oil with potassium hydroxide produced potassium soap with solid tangible yellowish white appearance and has a melting point 235 – 240 oC. Acidification potassium soap with hydrochloric acid was obtained a yellowish liquid fatty acid, density of 0.93 g.mL-1, refractive index 1.46 (25 º C), viscosity 60.54 cSt, acid number 140.55, saponification number 165.55, and ester number 25.00. Transesterification of sesame seed oil using methanol catalyzed potassium hydroxide produced methyl ester liquid fatty acids yellow colored, density of 0.89 g.mL-1, refractive index 1.45 (25 º C), viscosity 35.06 cSt, acid number 0.56, saponification number 429.21, and ester 428.65. Potassium soap (2% and 1%), fatty acids (2% and 1%), and methyl ester is 2% were active as an antibacterial agent to Escherchia coli and Staphylococcus aureus bacteria with medium category.
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