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Nanoparticles CaCO3/MgO by variation percent weight of MgO (5, 21, 22, 23, 24, and 25) wt.% potentially antibacterial can be synthesized by mixing methods and calcination at temperature 800 oC, which synthesis CaCO3 from shell fish (Anadara granosa) using carbonation methods by flow velocity of CO2 gas 2.8 liter/min. The successful nanoparticles were characterized by BET, antibacterial activity and PSA. Variation percent weight of MgO was used to determine pore size, surface area and antibacterial activity on nanoparticles CaCO3/MgO. The results showed that weight percent variation of MgO have different pore size and surface area that are included in the mesoporous category. CaCO3/MgO (5wt.%) has the smallest surface area (3.24 m2/g) and the largest pore size (20.12-29.35 nm), while CaCO3/MgO (24wt.%) has the largest surface area (65.05 m2/g) and the smallest pore size (3.23-3.59 nm) by the closed end of curve. The tip end of curve obtained at the sample CaCO3/MgO (21wt.%) which has the largest antibacterial activity by inhibitor diameter 31.0 mm on Staphylococcus aureus and 33.0 mm on Escherichia coli bacteria. The optimal nanoparticles has particle size <200 nm.