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Learning the materials about Animal Kingdom tends to be memorizing activities. Students assume that the materials are difficult to understand because the characteristics of this materials are descriptive and terms of complex anatomy and morphological structures. In order to eliminate the students- negative paradigm regarding the material about the Animal Kingdom, this research on instructional materials has been carried out to support 21st century skills. This research aimed to obtain relevant instructional materials, which was prepared based on students- prior knowledge. Research and Development method was used to develop this learning materials. This learning material is made based on students- prior knowledge and developed in accordance with propositions from the main references. The trial was conducted on 30 tenth-grade students. The result showed that students as the user of these learning materials were interested in invertebrate topic and also the exercises. The integration of learning materials and computer-based quiz are important to facilitate students- meaningful learning in 21st century.