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Menopause is a condition where a woman lost protection from estrogen, causing susceptibility to cardiovascular disease. Such condition is caused by narrowing of arterial lumen induced by increased Reactive Oxygen Species (ROS) activity and inflammation. It was reported that nicotine can reduce ROS production and act as anti-inflammation agent. Therefore, we conducted a research to explore the possibility of using nicotine to prevent arterial wall thickening and to reduce free radical activity. We did in vivo true experimental and randomized post-test only controlled group research in 18-20 weeks old Rattus norvegicus. We examine tail arterial wall thickness, lumen radius, and Wall to Lumen Ratio (WTLR), as well as Malondialdehyde (MDA) level as marker of ROS. The first group was normal rats (Sham). The second group represent ovariectomy group (ovariectomy-only/OVX). The third to fifth group was ovariectomized and given nicotine in dose of 0.25 mg/kg body weight (P1), 0.5 mg/kg (P2), and 0.75 mg/kg (P3). Group P1-P3 was given daily nicotine by oral route for 4 weeks, started from 3 weeks post ovariectomy. After treatment period was completed, needed organ was harvested. MDA levels of P3 were significantly lower than Ovx group (p = 0.036, Tukey HSD). P2 groups showed significantly thinner arterial wall (intimal-medial layer) than the Ovx group (p = 0.001, Tukey HSD). No significant different in lumen radius between groups was observed. WTLR measurement showed that P1, P2, and P3 has 6%, 42%, and 3% lower WTLR compared to Ovx group, respectively. Finally, we conclude that nicotine administration can prevent tail arterial wall thickening and has antioxidant Property.