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This research aims to find out the pre-service teacher-s physics attitude towards physics laboratory in one of the Universities in Aceh. The research method uses a quantitative descriptive approach. Data collection techniques used a scale questionnaire that was adapted from several previous researchers. The Subject of this study is 45 physics student prospective students were taken from the semester, II, IV, and IV as subjects of the study. The data analysis technique is done by calculating the mean of each aspect and indicator and the independent t-test to see differences in attitudes based on gender. Overall, the results of the study showed a negative attitude towards physical practicum activities with the acquisition of a mean of 2.78. The statistical test results for normally distributed and homogeneous data show no significant differences (df. 43, sig. 0.90> 0.04) between the attitudes of male and female students. Negative attitudes towards physics laboratory are caused by the design of practicum verification that do not provide benefits to improve understanding, practice scientific inquiry skills, thinking skills, and solving physics problems. Efforts to improve attitudes toward physics practicum can be done through the design of practical activities oriented to humanistic psychology and constructivism by involving students actively investigating. It is expected that the physics study lecturers to evaluate the practicum program not only suppress the understanding of concepts, but the skills aspects of conducting scientific investigations and attitudes also need to be provided to students through practical activities.