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Introduction Osteoarthritis (OA) due to predispose and biomechanics factors leads to direct degradation of the cartilage. This degradation process breakdowns the lipopolysaccharide of macrophages which then increases leukotrienes- production and plasmatic leukocyte. The red ginger (Z. officinale) and cogon grass (I. cylindrica) rhizomes are known to have an anti-inflammatory and antioxidant properties. This present study evaluated the effects of Z. officinale and I. cylindrica decoction on plasmatic leukocytes and its differential count in osteoarthritic-induced Wistar rats. Materials and methods Twenty five male Wistar rats aged four months, weighed 270-300 g were grouped into five groups; control (CTL), osteoarthritic rats (OA), and 3 different doses of treatment groups (P1, P2, P3). Each rat of OA and treatment groups were given 0,1 ml right knee intraarticular complete Freund-s adjuvant (CFA) injection to induce local osteoarthritic state. All treatment groups were orally administered with 40 mg/day of I. cylindrica decoction. However, P1, P2 and P3 groups were given 9, 18 and 36 mg of Z. officinale decoction each day for 10 days, respectively. Plasmatic leukocytes and its differential count were measured using hematology analyzer. Data were statistical analyzed with One Way ANOVA test followed by post hoc test. The significant level at P less than 0.05. Results The plasmatic leucocytes was 11.46 ± 7.72, 53.84 ± 22.39, 4.44 ± 1.04, 3.76 ± 0.35, and 8.94 ± 4.66 respectively in CTL, OA, P1, P2, and P3 (103/µL). The treatment groups have lower plasmatic leucocytes in comparison with OA group (P <0.05). We then evaluated the differential types of leucocyte; granulocyte, lymphocyte and monocyte. Plasmatic granulocyte was 2.03 ± 1.42, 13.7 ± 9.05, 0.21 ± 0.08, 0.47 ± 0.74, and 0.95 ± 1.11 respectively in CTL, OA, P1, P2, and P3 (103/µL). Plasmatic lymphocyte was 7.88 ± 5.43, 30 ±13.03, 3.14 ± 0.62, 2.9 ±0.34 and 6.6 ± 3.95 respectively in CTL, OA, P1, P2, and P3 (103/µL). Plasmatic monocyte was 1.72 ± 0.78, 8.12 ± 4.14, 1.08 ± 0.43, 0.74 ± 0.13 and 1.38 ± 0.63 respectively in CTL, OA, P1, P2, and P3 (103/µL). The treatment groups have lower differential count of leucocytes in comparison with OA group (P <0.05). Conclusions Decoction extract of Z officinale and I cylindrica reduced plasmatic leukocytes and corrected its differential count in osteoarthritic-induced Wistar rats. Combination of Z officinale and I cylindrica may have potential as anti-osteoarthritis. Keywords: Zingiber officinale, Imperata cylindrica, leucocytes, osteoarthritis *Correspondence: Dr. dr. Doti Wahyuningsih, M. Kes Faculty of Medicine University of Islam Malang Address: Jl. MT. Haryono 193 Malang, East Java province, Indonesia 65145 E mail address: dotiwahyuningsih@unisma.ac.id *Correspondence: dr.Rima Zakiyah,Sp.Rad Faculty of Medicine Uniersity of Islam Malang Address : Perum. Galxy Regency,Malang, East Java province,Indonesia