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The development of alternative energy sources from renewable natural resources has been carried out regulerly. One of the alternatives is the utilization of agricultural waste such as rice husk to produce bioethanol. However the cellulose content in rice husk is blocked by complex lignoseulosic matrices such as lignin which will disrupt the hydrolysis process. The pretreatment is needed to break the bond. In this study, pretreatment was carried out using the Aqueous Ammonia Soaking (AAS) and dilute acid methods with various ammonia concentration and temperature of pretreatment. The composition of rice husk was analyzed using Chesson methods. Three series of works were carried out, namely pretreatment, hydrolysis and fermentation. Pretreatment was carried out with various concentration of 5, 10, 15, 20, 25 % (v/v) and temperature of 60, 70, 80 90, 100 oC. The highest cellulose content was about 57.94% at ammonia concentration of 20% (v/v) and a temperature of 100 oC. Furthermore, enzymatic hydrolysis produced the highest glucose of about 24.1774 ppm and bioethanol of 11,21%.