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Black-winged myna (Acridotheres melanopterus) is an endemic species from Indonesia. Nowadays, the bird can be found only in Java and Bali islands, and it is important to make sure that the breeding centre is effectively breeding the bird to help the decreasing population wilderness. There is no difference between male and female based on the morphological characteristic. It can be a problem for the breeders to identify the sex when they have to pair the bird. The aim of this research is to identify the differences between male and female black-winged myna based on morphometric characteristic. There are weight, length, width, circumference, and the spread of the wings. The data is processed using principal component analysis (PCA) on the SPSS 20 program. The samples are 71 black-winged myna with minimum two years of age from breeding facilities in Java and Bali islands. Based on the research, morphometric characteristic that distinguishes male and female bird mostly is their wingspan and their weight. Morphometric characteristics of the female myna are smaller than the male. This result is essential for breeders to minimize the risk of pairing the same sex bird.