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Early pregnancy diagnostic is very important to support the attempt of fauna preservation. Lowland anoa is an endangered endemic species for Sulawesi Island and Buton. This species has natural wild characteristic and aggressive, intensified with silent heat effect which often occurs in this lowland anoa, makes it hard to detect its estrous cycle and pregnancy. The research is aimed to observe and morphology changing in pregnancy, and to find out diagnostic accuracy in pregnancy of lowland anoa using abdominal ultrasonography. Ultrasonography abdominal checking was used with 95% alcohol as transduction medium. Ultrasonography method was performed before mating and at day-21, day-191, and day-218 post natural mating using one dwarf female buffalo of 9 years old. Result showed that lowland anoa act was changing in 2nd after mating until 6th month before giving birth. Their morphological changing had shown in 3rd month, 6th month and 8th. In the 9th month the milk udder has enlarged. Based on the result of ultrasonography on day-21, it is very difficult to find the gestational sac. However, on day-191, the fetus spine, head, heart and lungs are clearly visible.