Indonesia Conference Directory

Pollen dispersal can be used to design and manage seed orchards of Mahogany to fit the breeding demand. Pollen dispersal and mating system in mahogany can be elucidated through pollen dispersal pattern analysis based on genetic structure. Microsatellite is a co-dominant genetic marker that has been widely applied for DNA analysis. The objective is this work were to to determine specific primers for parentage analysis in mahogany, to calculate the distance of pollens travel from donor pollen to female recipient, to estimate the frequency of pollination, and to evaluate the proportion of selfing and outcrossing in mahogany. The field activity was done by collecting leaf samples at genetic resource area of Sulawesi BPTH, South Sulawesi, Indonesia. The laboratory activities for genetic molecular analysis were conducted at Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Hasanuddin University, Indonesia. Results: The study indicated that the suitable primers for parentage analysis were sm05, sm18, and sm48. Pollens could travel up to 83 m apart from the evaluated female parents, and the highest frequency of pollination was 22 at 20 to 30 m. The proportion of outcrossing was 95% which indicated that mahogany trees in this evaluated area tend to outcross pollinate. Highly variable microsatellite markers have facilitated a direct genetic approach to measuring gene flow based on parentage analysis.