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Cellulases are a group of hydrolytic enzymes capable of hydrolyzing the cellulose to smaller sugar components produced by microorganisms during their growth. In this study, cellulose- degrading microorganism was isolated for obtaining effective cellulases from lignocellulosic biomass such as sugarcane bagasse and oil palm trunk waste from plantation area in Indonesia. A total of 194 strains were isolated on agar plates containing carboxymethyl cellulase (CMC). Of the 194 strains, 57 showed hydrolyzing zones on agar plates containing CMC after Congo-red staining. Cellulase assay kit (CellG5 method) was used to measure the enzyme activity of the obtained isolates. Two isolates among the 57 strains i.e. KS 30 and AT 17 showed higher carboxymethyl cellulase (CMCase) activity and isolate KS 30 exhibited the highest CMCase activity (1.891 Unit/mL). The strain KS-30 was identified as Bacillus subtilis on the basis of 16S rRNA gene sequence analysis. This study provided information about the prospect of cellulose-degrading Bacillus subtilis for the bioconversion of lignocellulosic biomass to produce value-added bioproducts such as ethanol, organic acids and other chemicals.